Translational_Unit
Part:BBa_K1641009:Design
Designed by: Pai Li Group: iGEM15_SYSU_CHINA (2015-09-12)
Fusion protein of Vika-EGFP-ssra, with RBS at beginning
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Ssra-Tag: for efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Dre is a Cre-like recombinase, so C-term EGFP fusion will not significant reduce its activity.
Source
Dre from BBa_K1641002 and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks.